fitc labeled anti cd34  (Elabscience Biotechnology)

Journal: Frontiers in Veterinary Science

Article Title: Evaluation of stability and safety of equine mesenchymal stem cells derived from amniotic fluid for clinical application

doi: 10.3389/fvets.2024.1330009

Figure Lengend Snippet: Isolation of equine amniotic fluid-derived stem cells and confirmation of differentiation ability. Equine amniotic fluid collection (A) . Morphology of cell isolated from equine amniotic fluid at passage 7. The MSC presenting a fibroblast-like characteristics and adherence to the plastic. The AFMSC was demonstrated under optical microscope in x100 (B) . The differentiation of AF-MSC into tri-lineages at passage 4. The adipogenic, chondrogenic, and osteogenic differentiation was confirmed by oil red O, alcian blue, and alizarin red S, respectively. The differentiation uninduced AF-MSC was not stained with oil red O [x100, (C) ], alcian blue [x100, (D) ], alizarin red S [x100, (E) ], and induced AF-MSC was stained with oil red O representing oil droplets [x100, (F) ], alcian blue representing connective tissue and cartilage matrix [x100, (G) ], alizarin red S representing matrix calcium formation [x100, (H) ].

Article Snippet: Cells were fixed with 4% paraformaldehyde (Thermo Fisher Scientific, MA, USA), and stained with antibodies against CD29 (phycoerythrin [PE]-labeled anti-human CD29 antibody; BioLegend, San Diego, CA, USA), CD44 (PE-labeled anti-mouse/human CD44 antibody; BioLegend), CD90 (PE-labeled mouse anti-rat CD90/mouse CD90.1; BD Biosciences), CD105 (fluorescein isothiocyanate [FITC]-labeled mouse anti-human CD105; Bio-Rad, Hercules, CA, USA), CD14 (porcine/equine CD14 antibody, R&D Systems, Minneapolis, MM, USA), CD34 (FITC-labeled mouse anti-human CD34; BD Pharmigen, Franklin Lakes, NJ, USA), CD45 (FITC-labeled mouse anti-human CD45; Southern Biotech, Birmingham, AL, USA), and major histocompatibility class II (FITC-labeled MHC class II antibody, clone CVS20; LSBio, Seattle, WA, USA).

Techniques: Isolation, Derivative Assay, Microscopy, Staining

Journal: Frontiers in Veterinary Science

Article Title: Evaluation of stability and safety of equine mesenchymal stem cells derived from amniotic fluid for clinical application

doi: 10.3389/fvets.2024.1330009

Figure Lengend Snippet: Stem cell marker variation. Relative gene expression level was normalized by expression level of target gene of skin fibroblast (A) . The graph and error bar represent mean of ΔCT value ± SEM. Significant difference represented * ( p = 0.0016) and ** ( p < 0.001). Cell surface marker analysis of AF-MSC at passage 5 to 7 by FACS (B) . The AF-MSC were positive for CD29, CD44, CD90, and CD105, and negative for CD14, CD34, CD38, CD45, and MHC class II. Expression of IgG was used for negative control.

Article Snippet: Cells were fixed with 4% paraformaldehyde (Thermo Fisher Scientific, MA, USA), and stained with antibodies against CD29 (phycoerythrin [PE]-labeled anti-human CD29 antibody; BioLegend, San Diego, CA, USA), CD44 (PE-labeled anti-mouse/human CD44 antibody; BioLegend), CD90 (PE-labeled mouse anti-rat CD90/mouse CD90.1; BD Biosciences), CD105 (fluorescein isothiocyanate [FITC]-labeled mouse anti-human CD105; Bio-Rad, Hercules, CA, USA), CD14 (porcine/equine CD14 antibody, R&D Systems, Minneapolis, MM, USA), CD34 (FITC-labeled mouse anti-human CD34; BD Pharmigen, Franklin Lakes, NJ, USA), CD45 (FITC-labeled mouse anti-human CD45; Southern Biotech, Birmingham, AL, USA), and major histocompatibility class II (FITC-labeled MHC class II antibody, clone CVS20; LSBio, Seattle, WA, USA).

Techniques: Marker, Expressing, Negative Control

Journal: International Journal of Molecular Sciences

Article Title: Impact of Double-Stranded RNA Internalization on Hematopoietic Progenitors and Krebs-2 Cells and Mechanism

doi: 10.3390/ijms24054858

Figure Lengend Snippet: Cytologic evaluation of internalization of FAM-dsRNA and TAMRA-dsDNA fragments into CD34+ Krebs-2 cells. ( a ) Krebs-2 cells treated with FAM, TAMRA-dUTP, FAM-dsRNA, and TAMRA-dsDNA. ( b ) Krebs-2 cells treated with: (1) FITC-labeled anti-CD34 antibodies (green) and TAMRA-dsRNA (red), (2) FAM-dsRNA (green) and PE-labeled anti-CD34 antibodies (red), and (3) FAM-dsRNA (green) and TAMRA-dsDNA (red). Arrows indicate stained cells. Flow cytometry data for the treated cell specimens are shown on the right-hand-side inset. ( c ) Microscopy analysis characterizing the dynamics of FAM-dsRNA accumulation in the same Krebs-2 cell. The images were recorded 5, 10, 20, 40, and 60 min after the labeled probe was added to the medium. The plots in the left corner of the images characterize the FAM intensity along the analyzed line (shown with a red arrow). ( d ) The diagram showing the dynamics of FAM-dsRNA accumulation in Krebs-2 cells.

Article Snippet: In some experiments, after the incubation with labeled probes, cells were stained with PE-labeled anti-CD34 antibodies (BD Biosciences, San Jose, CA, USA) or FITC-labeled anti-CD34 antibodies (Sony Biotechnology, San Jose, CA, USA) according to the manufacturer’s protocol in the dark at room temperature for 30 min.

Techniques: Labeling, Staining, Flow Cytometry, Microscopy